Descrição
*** SOLICITE SEU ORÇAMENTO A LIVRARIA BIOTEC – EMAIL.: biotec@livrariabiotec.com.br – CNPJ 07.993.228/0001-79 *** PREÇO SUJEITO A REAJUSTE
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Contents
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VOLUME 1 Part 1: Essentials
- 1. Isolation and Quantification of DNA
- 2. Analysis of DNA
- 3. Cloning and Transformation with Plasmid Vectors
- 4. Gateway Recombinational Cloning
- John S. Reece-Hoyes and Albertha J.M. Walhout
- 5. Working with Bacterial Artificial Chromosomes and Other High-Capacity Vectors
- Nathaniel Heintz and Shiaoching Gong
- 6. Extraction, Purification, and Analysis of RNA from Eukaryotic Cells
- 7. Polymerase Chain Reaction
- 8. Bioinformatics
- Jui-Hung Hung and Zhiping Weng
- VOLUME 2
- Part 2: Analysis and Manipulation of DNA and RNA
- 9. Quantification of DNA and RNA by Real-Time Polymerase Chain Reaction
- 10. Nucleic Acid Platform Technologies
- Oliver Rando
- 11. DNA Sequencing
- Elaine Mardis and W. Richard McCombie
- 12. Analysis of DNA Methylation in Mammalian Cells
- Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee
- 13. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes
- 14. Methods for In Vitro Mutagenesis
- Matteo Forloni, Alex Liu, and Narendra Wajapeyee
- Part 3: Introducing Genes into Cells
- 15. Introducing Genes into Cultured Mammalian Cells
- Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil
- 16. Introducing Genes into Mammalian Cells: Viral Vectors
- Guangping Gao and Miguel Sena-Esteves
- VOLUME 3
- Part 4: Gene Expression
- 17. Analysis of Gene Regulation Using Reporter Systems
- Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar
- 18. RNA Interference and Small RNA Analysis
- Chengjian Li and Phillip D. Zamore
- 19. Expressing Cloned Genes for Protein Production, Purification, and Analysis
- Clara L. Kielkopf, William Bauer, and Ina Urbatsch
- Part 5: Interaction Analysis
- 20. Cross-Linking Technologies for Analysis of Chromatin Structure and Function
- Tae Hoon Kim and Job Dekker
- 21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking Immunoprecipitation (CLIP)
- Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B. Darnell
- 22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays
- John S. Reece-Hoyes and Albertha J.M. Walhout
- Appendices
- 1. Reagents and Buffers
- 2. Commonly Used Techniques
- 3. Detection Systems
- 4. General Safety and Hazardous Material
- Index
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- Paperback : 2000 pages
- ISBN-10 : 1936113422 – ISBN 13 : 9781936113422
