PLANT SECONDARY METABOLISM ENGINEERING – METHODS AND APPLICATIONS

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CONTENTS.:

1 Plant Secondary Metabolism and Challenges in Modifying Its Operation: An Overview Naíla Cannes do Nascimento Arthur Germano Fett-Neto  2 Suppression Subtractive Hybridization as a Tool to Identify Anthocyanin Metabolism-Related Genes in Apple Skin Yusuke Ban Takaya Moriguchi  3 Identification of Regulatory Protein Genes Involved in Alkaloid Biosynthesis Using a Transient RNAi System Yasuyuki Yamada Nobuhiko Kato Yasuhisa Kokabu Qingyun Luo Joseph Gogo Dubouzet Fumihiko Sato  4 Site-Directed Mutagenesis and Saturation Mutagenesis for the Functional Study of Transcription Factors Involved in Plant Secondary Metabolite Biosynthesis Sitakanta Pattanaik Joshua R. Werkman Que Kong Ling Yuan  5 Isolation of Proteins Binding to Promoter Elements of Alkaloid Metabolism-Related Genes Using Yeast One-Hybrid Débora Vom Endt Giancarlo Pasquali  6 Modulation of Carotenoid Accumulation in Transgenic Potato by Inducing Chromoplast Formation with Enhanced Sink Strength Joyce Van Eck Xiangjun Zhou Shan Lu Li Li  7 Over-expression of Rate-Limiting Enzymes to Improve Alkaloid Productivity Tomoya Takemura Yit-lai Chow Takehiko Todokoro Takuya Okamoto Fumihiko Sato  8 Microbial Expression of Alkaloid Biosynthetic Enzymes for Characterization of Their Properties Hiromichi Minami Nobuhiro Ikezawa Fumihiko Sato  9 Producing a Recombinant Flavin-Containing Monooxygenase from Coffea arabica in Escherichia coli for Screening of Potential Natural Substrates Igor Cesarino Paulo Mazzafera 10 Efficient Production of Active Form Recombinant Cassava Hydroxynitrile Lyase Using Escherichia coli in Low-Temperature Culture Hisashi Sembu Eita Ichige Tadayuki Imanaka Haruyuki Atomi Hideki Aoyagi 11 Introduction of the Early Pathway to Taxol Biosynthesis in Yeast by Means of Biosynthetic Gene Cluster Construction Using SOE-PCR and Homologous Recombination Pia Dahm Stefan Jennewein 12 Biocatalytic Synthesis of Tritium (3H)-Labelled Taxa-4(5),ll(12)-diene, the Pathway Committing Precursor of the Taxoid Diterpenoids Hans Schmeer Stefan Jennewein 13 USER Cloning and USER Fusion: The Ideal Cloning Techniques for Small and Big Laboratories Hussam H. Nour-Eldin Fernando Geu-Flores Barbara A. Halkier 14 Enrichment of Carotenoids in Flaxseed by Introducing a Bacterial Phytoene Synthase Gene Masaki Fujisawa Noribiko Misawa 15 Metabolic Engineering by Plastid Transformation as a Strategy to Modulate Isoprenoid Yield in Plants Tomohisa Hasunuma Akihiko Kondo Chikahiro Miyake 16 Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes Victor P. Bulgakov Yuri N. Shkryl Galina N. Veremeichik 17 Flow Cytometric Methods to Investigate Culture Heterogeneities for Plant Metabolic Engineering Vishal Gaurav Martin E. Kolewe Susan C. Roberts 18 Phenylpropanoid Biosynthesis in Leaves and Glandular Trichomes of Basil (Ocimum basilicum L.) Cícero Deschamps James E. Simon 19 Fusion with Fluorescent Proteins for Subcellular Localization of Enzymes Involved in Plant Alkaloid Biosynthesis Patrícia Duarte Johan Memelink Mariana Sottomayor 20 Immunohistochemical Localisation of a Putative Flavonoid Transporter in Grape Berries Elisa Petrussa Enrico Braidot Marco Zancani Carlo Peresson Alberto Bertolini Sonia Patui Valentino Casolo Sabina Passamonti Francesco Macrì Angelo Vianello 21 Electrogenic Bromosulfalein Transport in Isolated Membrane Vesicles: Implementation in Both Animal and Plant Preparations for the Study of Flavonoid Transporters Sabina Passamonti Federica Tramer Elisa Petrussa Enrico Braidot Angelo Vianello Subject Index 337

por Arthur Germano Fett-Neto (Editor) – 2010

  • Capa dura ‏ : ‎ 339 paginas
  • ISBN-10 ‏ : ‎ 1607617226
  • ISBN-13 ‏ : ‎ 9781607617228

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Peso 2 kg