Descrição
CONTENTS:
Project I: Genomic DNA Isolation and Analysis
Module I.1 Mouse Genomic DNA Isolation and Analysis
Module I.2 Southern Blot Experiment to Detect Gene Specific Sequences in Genomic DNA
Module I.3 PCR Amplify a Specific Gene from Mouse Genomic DNA
Project II: Genomic Cloning, DNA Sequencing and Bioinformatics
Module II.1 Prepare Mouse Genomic DNA for Cloning, Production and Titering of a Lambda Phage Genomic Library
Module II.2 Screen a Lambda Phage Library to Detect Clones of a Specific Gene
Module II.3 PCR Subclone into a Plasmid Vector, Propagate in Bacterial Cells, and Prepare Purified Recombinant Plasmid DNA
Module II.4 Dideoxy Thermal Cycle DNA Sequencing of a Cloned Gene
Module II.5 Bioinformatic Analysis of the DNA Sequence Data
Project III: Gene Expression Analysis: RNA Isolation, Northern Blot, and Reverse Transcriptase-Polymerase Chain Reaction
Module III.1 Isolation and Analysis of Total RNA from Mouse Liver
Module III.2 Northern Blot Experiment to Detect a Specific Gene Transcript
Module III.3 Reverse Transcriptase-Polymerase Chain Reaction Amplification of a Specific Gene Transcript
Project IV: cDNA Cloning and cDNA Library Analysis
Module IV.1 Construction of a cDNA Library and cDNA Library Analysis
Module IV.2 Characterization and Screening of a cDNA Library Using the Polymerase Chain Reaction
Appendix I: Available Kits and Componenets
Appendix II: General Molecular Biology Laboratory Set Up
Appendix III: Marker Maps and Sizes
Appendix IV: Alternate and Additional Protocols
Appendix V: Other Useful Protocols from RT-PCR Reactions
Appendix VI: Useful Calculations
Appendix VII: References, Helpful Books, and Additional Resour
