MOLECULAR CLONING – 4/ED 2012 – 3 VOLS – PAPER

Descrição

*** SOLICITE SEU ORÇAMENTO A LIVRARIA BIOTEC – EMAIL.: biotec@livrariabiotec.com.br  – CNPJ 07.993.228/0001-79 ***  PREÇO SUJEITO A REAJUSTE

• Contents

• VOLUME 1 Part 1: Essentials

  1. Isolation and Quantification of DNA

  2. Analysis of DNA

  3. Cloning and Transformation with Plasmid Vectors

  4. Gateway Recombinational Cloning

  John S. Reece-Hoyes and Albertha J.M. Walhout

  5. Working with Bacterial Artificial Chromosomes and Other High-Capacity Vectors

  Nathaniel Heintz and Shiaoching Gong

  6. Extraction, Purification, and Analysis of RNA from Eukaryotic Cells

  7. Polymerase Chain Reaction

  8. Bioinformatics

  Jui-Hung Hung and Zhiping Weng

  VOLUME 2

  Part 2: Analysis and Manipulation of DNA and RNA

  9. Quantification of DNA and RNA by Real-Time Polymerase Chain Reaction

  10. Nucleic Acid Platform Technologies

  Oliver Rando

  11. DNA Sequencing

  Elaine Mardis and W. Richard McCombie

  12. Analysis of DNA Methylation in Mammalian Cells

  Paul M. Lizardi, Qin Yan, and Narendra Wajapeyee

  13. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes

  14. Methods for In Vitro Mutagenesis

  Matteo Forloni, Alex Liu, and Narendra Wajapeyee

  Part 3: Introducing Genes into Cells

  15. Introducing Genes into Cultured Mammalian Cells

  Priti Kumar, Arvindhan Nagarajan, and Pradeep D. Uchil

  16. Introducing Genes into Mammalian Cells: Viral Vectors

  Guangping Gao and Miguel Sena-Esteves

  VOLUME 3

  Part 4: Gene Expression

  17. Analysis of Gene Regulation Using Reporter Systems

  Pradeep D. Uchil, Arvindhan Nagarajan, and Priti Kumar

  18. RNA Interference and Small RNA Analysis

  Chengjian Li and Phillip D. Zamore

  19. Expressing Cloned Genes for Protein Production, Purification, and Analysis

  Clara L. Kielkopf, William Bauer, and Ina Urbatsch

  Part 5: Interaction Analysis

  20. Cross-Linking Technologies for Analysis of Chromatin Structure and Function

  Tae Hoon Kim and Job Dekker

  21. Mapping of In Vivo RNA-Binding Sites by UV-Cross-Linking Immunoprecipitation (CLIP)

  Jennifer C. Darnell, Aldo Mele, Ka Ying Sharon Hung, and Robert B. Darnell

  22. Gateway-Compatible Yeast One-Hybrid and Two-Hybrid Assays

  John S. Reece-Hoyes and Albertha J.M. Walhout

  Appendices

  1. Reagents and Buffers

  2. Commonly Used Techniques

  3. Detection Systems

  4. General Safety and Hazardous Material

  Index

  • Paperback ‏ : ‎ 2000 pages
  • ISBN-10 ‏ : ‎ 1936113422 – ISBN 13 : 9781936113422