DROSOPHILA CELLS IN CULTURE

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  1. Contents
  2. Introduction
  3. Chapter 1. Composition of the Body Fluid of Drosophila and the Design of Culture Media for Drosophil
  4. I. Main Characteristics of the “Milieu Intérieur” of Drosophila
  5. II. Description and Comparison of the Main Culture Media Available
  6. III. Buffered Saline Solutions for Drosophila Tissues
  7. IV. General Discussion
  8. V. Prospects
  9. Appendix 1
  10. References
  11. Chapter 2. Primary Cell Cultures of Drosophila Cells
  12. I. Setting up Primary Cultures
  13. II. Evolution of Primary Cultures from Disaggregated Wild-Type Embryos: Characteristics of Main Cell
  14. III. Primary Cultures of Muscle Cells
  15. IV. Nerve Cells in Primary Cultures
  16. V. In Vitro Differentiation of Imaginal Cells: Hormonal Control
  17. VI. Cell Cultures from Embryonic (Lethal) Mutants
  18. Appendix 2
  19. References
  20. Chapter 3. Drosophila Continuous Cell Lines
  21. I. Establishing Embryonic Cell Lines
  22. II. Maintenance of Cell Lines
  23. III. Catalog of Established Drosophila Embryonic Cell Lines
  24. IV. Cell Lines from Larval or Imaginal Tissues
  25. V. Cell Counting and Viability Determination
  26. VI. Cell Cloning
  27. VII. Storage by Freezing
  28. VIII. Cell Fusion and Selection of Somatic Hybrids
  29. IX. Living Cell Transportation
  30. Appendix 3
  31. References
  32. Chapter 4. Karyotype and Cell Cycle
  33. I. Karyotype Analysis
  34. II. Cell Growth and Division Cycle
  35. III. Haploid Cell Lines and Acentriolar Status of One of Them
  36. Appendix 4
  37. References
  38. Chapter 5. Biology and Biochemistry of Cultured Cell Lines: 1. Nucleic Acids
  39. I. Nucleic Acid Metabolism: Biosynthesis of Nucleoside Triphosphates
  40. II. Ribosomal RNAs
  41. III. Mitochondrial Genome
  42. IV. DNA Repair and Sensitivity to Mutagens
  43. V. Transcription Apparatus and Regulatory Factors
  44. Appendix 5
  45. References
  46. Chapter 6. Biology and Biochemistry of Cultured Cell Lines: 2. Proteins
  47. I. Cell Surface Glycoproteins: Adhesion/Recognition Molecules
  48. II. Growth Factors, Receptors, and Protooncogenes
  49. III. Drosophila Humoral Defenses
  50. IV. Other Proteins or Enzymatic Activities
  51. References
  52. Chapter 7. Experimental Models of Gene Regulation: 1. Heat-Shock Response of Drosophila Cells
  53. I. The Heat-Shock System
  54. II. Major Heat-Shock Proteins and Their Encoding Genes
  55. III. Heat-Shock Protein Induction at Transcriptional Level
  56. IV. Translational Control in Heat Shock
  57. V. Recovery from Heat Shock
  58. VI. Other Temperature-Inducible Genes
  59. VII. Cell Responses to Different Stresses
  60. VIII. Intracellular Localizations and Putative Functions of Heat-Shock Proteins
  61. IX. Regulatory Regions of hsp Genes and Transcriptional Activators
  62. References
  63. Chapter 8. Experimental Models of Gene Regulation: 2. Cell Responses to Hormone
  64. I. Hormonal Stimulus
  65. II. Repertoire of Cell Responses
  66. III. “Maturation” Phase: A Critical Period for Hormone Action
  67. IV. Cell Resistance to Ecdysteroids
  68. V. Ecdysteroid Receptors
  69. VI. Functional Interference with Juvenile Hormone
  70. VII. Validity of Model and Its Current Interest
  71. References
  72. Chapter 9. Gene Transfer into Cultured Drosophila Cells
  73. I. Cell Transformation
  74. II. Transient Expression
  75. III. Recipient Cell Lines
  76. IV. Transfection Methods
  77. V. Selection Systems
  78. VI. Efficient Promoters
  79. VII. Reporter Genes
  80. VIII. Shuttle Vectors
  81. IX. Prospects
  82. Appendix 9
  83. References
  84. Chapter 10. Transposons
  85. I. Long Terminal Repeat Retrotransposons: copia-like Elements
  86. II. Line-Like Retroposons
  87. III. Bacterial Insertion-like Transposons: P Element
  88. References
  89. Chapter 11. Drosophila Viruses and Other Infections of Cultured Cells
  90. I. Drosophila Hereditary Sigma Virus and Persistent Infection with Various Arboviruses
  91. II. Endogenous Viruses of Drosophila and Cell Lines
  92. III. Looking for Efficient Viral Promoters in Drosophila Cell Lines
  93. IV. Experimental Studies of Parasite–Host Cell Relations in Drosophila Cell Lines
  94. Appendix 11
  95. References
  96. Bibliography
  97. Index
  98. por Guy Echalier  (Autor) – 1997 
  99. Capa dura ‏ : ‎ 702 paginas
  100. ISBN-10 ‏ : ‎ 0122294602
  101. ISBN-13 ‏ : ‎ 9780122294600

 

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